細(xì)菌性腦膜炎3重核酸檢測試劑盒(PCR方法)

廣州健侖生物科技有限公司

準(zhǔn)備使用lyo master混合物（8孔條）檢測腦膜炎奈瑟菌（Nmeng），肺炎鏈球菌（Spneu）和流感嗜血桿菌（Haeinf），包括內(nèi)部對(duì)照。
Ready to use lyo master mix (8-well strips) for detection of Neisseria meningitidis (Nmeng), Streptococcus pneumoniae (Spneu) and Haemophilus influenzae (Haeinf) including an internal control.

細(xì)菌性腦膜炎3重核酸檢測試劑盒(PCR方法)

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細(xì)菌性腦膜炎3重核酸檢測試劑盒(PCR方法)

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

細(xì)菌、酵母菌，放線菌和霉菌；
肉膏蛋白胨斜面培養(yǎng)基，滅菌脫脂牛乳，滅菌水，化學(xué)純的液體石蠟，甘油，五氧化二磷，河沙，瘦黃土或紅土，冰塊、食鹽，干冰，95％酒精，10％鹽酸，無水氯化鈣；
滅菌吸管，滅菌滴管，滅菌培養(yǎng)皿，管形安瓿管，淚滴形安瓿管（長頸球形底），40目與 100目篩子，油紙，濾紙條（0．5×1．2cm），干燥器，真空泵，真空壓力表，噴燈，L形五通管，冰箱，低溫冰箱（-30℃），液氮冷凍保藏器。
四、操作步驟、各保藏法的應(yīng)用范圍及優(yōu)缺點(diǎn)
下列各法可根據(jù)實(shí)驗(yàn)室具體條件與需要選做。
1．斜面低溫保藏法
將菌種接種在適宜的固體斜面培養(yǎng)基上，待菌充分生長后，棉塞部分用油紙包扎好，移至2—8℃的冰箱中保藏。
保藏時(shí)間依微生物的種類而有不同，霉菌、放線菌及有芽孢的細(xì)菌保存2—4個(gè)月，移種一次。酵母菌兩個(gè)月，細(xì)菌每月移種一次。
此法為實(shí)驗(yàn)室和工廠菌種室常用的保藏法，優(yōu)點(diǎn)是操作簡單，使用方便，不需特殊設(shè)備，能隨時(shí)檢查所保藏的菌株是否死亡、變異與污染雜菌等。缺點(diǎn)是容易變異，因?yàn)榕囵B(yǎng)基的物理、化學(xué)特性不是嚴(yán)格恒定的，屢次傳代會(huì)使微生物的代謝改變，而影響微生物的性狀；污染雜菌的機(jī)會(huì)亦較多。
2．液體石蠟保藏法
（1）將液體石蠟分裝于三角燒瓶內(nèi)，塞上棉塞，并用牛皮紙包扎，1．05kg/cm2>，121．3℃滅菌30分鐘，然后放在40℃溫箱中，使水汽蒸發(fā)掉，備用。
（2）將需要保藏的菌種，在zui適宜的斜面培養(yǎng)基中培養(yǎng)，使得到健壯的菌體或孢子。
（3）用滅菌吸管吸取滅菌的液體石蠟，注入已長好菌的斜面上，其用量以高出斜面頂端1cm為準(zhǔn)（圖Ⅶ-12），使菌種與空氣隔絕。
Third, equipment
Bacteria, yeasts, actinomycetes and molds;
Cream peptone slant medium, sterilized skim milk, sterilized water, chemically pure liquid paraffin, glycerol, phosphorus pentoxide, river sand, thin yellow or red clay, ice, salt, dry ice, 95% Hydrochloric acid, anhydrous calcium chloride
Sterilizing pipette, sterilizing pipette, sterilizing petri dish, ampule tube ampule, teardrop ampule tube (long neck spherical bottom), 40 mesh and 100 mesh sieve, paper, filter paper (0.5 × 1. 2cm), dryer, vacuum pump, vacuum gauge, torch, L-shaped five-tube, refrigerator, low temperature refrigerator (-30 ℃), liquid nitrogen freezer.
Fourth, the operational steps, the scope and advantages and disadvantages of each deposit law
The following methods can be selected according to the specific conditions and needs of the laboratory.
1. Inclined surface temperature preservation method
The strain was inoculated on a suitable solid slant culture medium, to be fully grown bacteria, cotton tampon partially wrapped with oil paper, moved to 2-8 ℃ refrigerator preservation.
Storage time varies according to the type of microorganisms, molds, actinomycetes and spores of bacteria stored for 2-4 months, transplanted once. Yeast two months, the bacteria is best to move once a month.
This method is commonly used in laboratories and factory bacteria room preservation method, the advantages of simple operation, easy to use, without special equipment, can check the deposited strains at any time whether the death, mutation and contamination of bacteria. The disadvantage is that it is easy to mutate because the physical and chemical properties of the medium are not strictly constant. Repeated passages will change the metabolism of the microorganisms and affect the characteristics of the microorganisms. There is also more chance of contamination of the bacteria.
2. Liquid paraffin deposit method
(1) Dispense the liquid paraffin into a conical flask, tampon it, and wrap it with kraft paper 1.05kg / cm2> and sterilize it at 121.3 ° C for 30 minutes and then place it in a 40 ° C incubator to evaporate the water vapor Off, spare.
(2) The strain to be preserved, cultured in the most appropriate slant medium, so that robust bacteria or spores.
(3) The sterilized liquid paraffin is drawn in with a sterile pipette and injected into the slant surface which has grown well. The dosage is about 1cm above the top of the bevel (Figure VII-12), isolating the strain from the air.
(4) Keep the tube upright, store at low temperature or at room temperature (some microorganisms are longer than the refrigerator at room temperature).