戊型肝炎病毒檢測試劑盒（PCR-熒光探針法）

廣州健侖生物科技有限公司

單管多重用于檢測和定量戊型肝炎病毒和內(nèi)部對照。

One tube multiplex for detection AND quantification of hepatitis E virus and internal control.

戊型肝炎病毒檢測試劑盒（PCR-熒光探針法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

四、實(shí)驗(yàn)說明
大腸桿菌中除了不同型細(xì)胞的接合外，同型細(xì)胞F+與F+或*即濃度為使用濃度的50倍。
將稱好的藥品分別溶解于670毫升蒸餾水中，待一種藥品溶解后再放另一種藥品，直至全部藥品都溶解，然后加水定容到1.000毫升。
瓊脂的添加量由1.5—2克，根據(jù)瓊脂的質(zhì)量而定。凝固性能好的瓊脂，可少加一些，反之，則要多加一些。Hfr與Hfr也能接合，但重組頻率很低。細(xì)胞中的F因子使細(xì)胞壁的抗原發(fā)生了變化，這些不同于F性菌毛的表面成分阻止了F+與F+細(xì)胞雜交。經(jīng)培養(yǎng)后處于饑餓條件下的F+細(xì)胞喪失了它們的表面排斥性，才能與其它F+細(xì)胞接合（這種改變是暫時的，一旦在新鮮培養(yǎng)基中，繼續(xù)生長正常的表面特性又恢復(fù)），這些表型上的“F-細(xì)胞”稱為擬表型。在抑制新的F性菌毛和表面成分形成的條件下生長，如低溫下連續(xù)通氣培養(yǎng)24—48小時，能增加擬表型的百分?jǐn)?shù)。
1946年Lederberg和Tatum開始發(fā)現(xiàn)細(xì)菌的接合以后，普遍認(rèn)為DNA的轉(zhuǎn)移必需F+（Hfr）與F-細(xì)胞的緊密接觸。Anderson等于1957年根據(jù)電鏡照片指出接合細(xì)胞之間形成了橋。之后發(fā)現(xiàn)F+（Hfr）和性菌毛之間的關(guān)系，認(rèn)為細(xì)菌染色體和專一雄性噬菌體通過性菌毛而轉(zhuǎn)移。近來的電鏡照片已經(jīng)發(fā)現(xiàn)接合細(xì)胞通過性菌毛接觸，并有實(shí)驗(yàn)依據(jù)。細(xì)胞接合后，供體中的DNA開始合成。一個單鏈DNA轉(zhuǎn)移入受體并合成一條新的互補(bǔ)鏈；這過程能以DNA復(fù)制的滾環(huán)模型來說明。
上面提到帶有F因子的大腸桿菌有F+和Hfr二類，實(shí)際上并不是所有的Hfr全是相當(dāng)穩(wěn)定的，在許多Hfr群體中含有回復(fù)子，在這些回復(fù)子中F因子不再整合在染色體上，而回復(fù)到游離狀態(tài)，當(dāng)F因子脫離寄主染色體時攜帶了細(xì)菌的基因，例如lac和gal等，這稱之為F′因子。帶有F′因子的菌稱為F′菌株。F′菌株也能與F-雜交，現(xiàn)被廣泛應(yīng)用于細(xì)菌的研究工作。

Fourth, experimental instructions
In addition to the different types of cells in E. coli junction, the same type of cells F + or F + or * concentration of the concentration of 50 times.
Will be good drugs were dissolved in 670 ml of distilled water, until a drug dissolved after the release of another drug until all drugs are dissolved, and then add water to a fixed volume of 1.000 ml.
The amount of agar added by 1.5-2 grams, depending on the quality of agar. Good solidification agar, add less, on the contrary, will have to add more. Hfr and Hfr can also join, but recombination frequency is very low. F cell factor in the cell wall antigen has changed, these are different from the F component of the surface of the pili to prevent F + and F + cells hybridization. F + cells that are starved under culture lose their surface exclusivity after incubation in order to engage with other F + cells (this change is temporary, and resumes when the normal surface properties continue to grow in fresh medium) Phenotypic "F-cells" are referred to as being phenotypic. Growth in conditions that inhibit the formation of new F-type pili and surface components, such as continuous aeration at low temperatures for 24-48 hours, increases the percentage of the phenotype.
After Lederberg and Tatum began to discover bacterial conjugation in 1946, it was generally accepted that the transfer of DNA required close contact of F + (Hfr) with F-cells. Anderson et al., 1957, based on electron micrographs, point out the formation of bridges between the mating cells. After that, the relationship between F + (Hfr) and the sex pili was found, suggesting that the bacterial chromosome and the specific male phage are transferred through the sex pili. Recent electron micrographs have revealed that the conjunctival cells are contacted by the pili and have experimental evidence. After the cells are joined, the DNA in the donor begins to synthesize. A single-stranded DNA translocates into the receptor and synthesizes a new complementary strand; this process can be described as a rolling-loop model of DNA replication.
As mentioned above, F-containing E. coli has two classes of F + and Hfr. In fact, not all Hfr are quite stable, and in many Hfr populations contain a recovery factor in which F factor is no longer integrated Chromosomes, and returned to the free state, when the F factor off host chromosomes carrying bacterial genes, such as lac and gal, which is called F 'factor. F 'factor-bearing bacteria are called F' strains. F 'strains also hybridize with F-F, and are now widely used in bacterial research.